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Gram

Microbiology

For microscopical examination the sample is fixed and stained. The Gram staining is often the first step in the identification of a bacterial organism. While Gram staining is a valuable diagnostic tool in both clinical and research settings, not all bacterisa can be definitely classified by this technique.

Gram staining (or Gram's method) is an empirical method of differentiating bacterial species into two large groups (Gram positive and Gram negative) based on the chemical and physical properties of their cell walls. Gram-positive bacteria appears purple whereas the Gram-negative bacterial species appear pink in spinal fluid sample.

There are four basic steps of the Gram stain, which include applying primary stain (crystal violet) to a heat fixed smear of a bacterial culture, followed by the addition of a trapping agent (Gram's iodine or Lugol iodine solution), rapid decolorisation with acetone+spirit solution, and counterstanding with safranin. All of the necessary reagents are included in the Reagena Gram staining.

Product
 

Volume

Product code

Gram staining 

 

 

Acetone Spirit (1+1) Destainer

Crystal violet 2 %

 

Lugol's solution

 

Safranin solution 0.25 %

 

1 L

100 ml

1 L

100 ml

1 L

100 ml

1 L

180019

140191

180191

140197

180197

140331

180331

Related products
 

Kovac's reagent

Löffler's methylene blue solution 0.4% m/V

Methyl violet 0.25 % m/V

Cleaning solution for microscopes

100 ml

100 ml

100 ml

50 ml

500 ml

140184

140201

140216

132022

172022

 

 

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